Methods for Labeling a Population of RNA Molecules

专利名称：Methods for Labeling a Population of RNA

Molecules

发明人：Ira Schildkraut,Laurence Ettwiller,Ivan R.

Correa, Jr.,George Tzertzinis,JohnBuswell,Madalee G. Wulf

申请号：US15908522申请日：20180228

公开号：US20180195061A1公开日：20180712

摘要：A method of labeling, and optionally enriching, for a population of target RNAmolecules in a mixture of RNAs is provided. In some embodiments, the method maycomprise (a) adding a label to the 5′ end of 5′-diphosphorylated or 5′-triphosphorylatedtarget RNA molecules in a sample by incubating the sample with labeled GTP and acapping enzyme; and (b) optionally enriching for target RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag. The label may be anoligonucleotide, which may further comprise an affinity group attached either internallyor at 5′ or 3′ end of the oligonucleotide where the oligonucleotide label may be addeddirectly, or indirectly via a reaction with a reactive group to the target RNA.

申请人：New England Biolabs, Inc.

地址：Ipswich MA US

国籍：US

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